Pan paniscus raw sequence reads - SRA

SRX22045569: Pan paniscus raw sequence reads
1 OXFORD_NANOPORE (PromethION) run: 1.2M spots, 50.7G bases, 39.7Gb downloads

Design: The UHMW DNA was extracted from frozen cell pellets using the Circulomics Nanobind UHMW DNA Extraction Protocol and assessed for fragment size using a pulsed field gel. The fragments span from 50 Kb to 1000 Kb. Genomic DNA libraries were prepared using the "(ONT/CirculomicsLibraryProtocol)SQK-ULK001-ULK_9124_v110_revA_24Mar2021-promethion.pdf protocol from Oxford Nanopore. Each library was loaded onto a FLO-PRO002 flow cell and run for 72 hours with two subsequent loadings at 24-hour intervals. The libraries were sequenced on a PromethION (Oxford Nanopore) running MinKNOW software version 21.05.20. Basecalling and modified base detection (5mC) were performed using Guppy 6.3.8.

Submitted by: NIH/NHGRI

Study: Pan paniscus Raw sequence reads

PRJNA986878 • SRP448348 • All experiments • All runs

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This data was generated to assemble the Pan paniscus cell line PR00251 (ToLID: mPanPan1). The genome was sequenced with multiple technologies. PR00251's parents (mother=mPanPan2, father=mPanPan3) were also sequenced with Illumina to utilize trio markers.

Sample: bonobo

SAMN13935689 • SRS6111929 • All experiments • All runs

Organism: Pan paniscus

Library:

Name: BET0001_1_PAG24525

Instrument: PromethION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 1.2M spots, 50.7G bases, 39.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR26337639	1,162,256	50.7G	39.7Gb	2023-10-11

ID:: 29969307

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